A Chromatographic Separation of Bilirubin Glucuronides from Human Bile.
نویسندگان
چکیده
منابع مشابه
Separation by thin-layer chromatography and structure elucidation of bilirubin conjugates isolated from dog bile.
1. A system for separation of bile pigments by t.l.c. and for their structure elucidation is presented. Separated bile pigments are characterized by t.l.c. of derived dipyrrolic azopigments. 2. At the tetrapyrrolic stage hydrolysis in strongly alkaline medium followed by t.l.c. demonstrates the presence of bilirubin-IIIalpha, -IXalpha and -XIIIalpha and allows assessment of their relative amoun...
متن کاملThe isolation and characterization of bilirubin diglucuronide, the major bilirubin conjugate in dog and human bile.
The chemical structure of the major conjugate of bilirubin was unequivocally elucidated by structural analysis. The conjugated bilirubins were first separated from the lipid components of human duodenal aspirates or dog gall-bladder bile, and then resolved by t.l.c. into a series of tetrapyrroles. The major tetrapyrrole was then converted into its more stable dipyrrolic azo derivative for furth...
متن کاملIsolation and properties of conjugated bilirubin from bile.
1. A simple, rapid solvent partition method is described for isolation of conjugated bilirubin, free of unconjugated bilirubin, bile salts, phospholipids and cholesterol, from rat bile. Yields are 40-58%. The product is a phosphate-buffered solution containing approx. 0.4mg of bilirubin/ml, principally as mono- and di-glucuronide conjugates. The method may be modified for isolation of conjugate...
متن کاملInteractions of unconjugated bilirubin with bile salts.
The rate of peroxidation of unbound, unconjugated bilirubin (UCB) was used to assess the interactions of UCB with four taurine-conjugated bile salts at pH 8.2, 37 degrees C, and an ionic strength of 0.15. Each of the four structurally different bile salts markedly decreased the rate of peroxidation of UCB in the presence of horseradish peroxidase (HRP); 30% of UCB was bound even at low, premice...
متن کاملThe Chromatographic Separation of Bile Acids I. the Separation of Cholic and Desoxycholic Acid by Henryk Silberman
The physiological and industrial importance of cholic and desoxycholic acids, the two main constituents of the bile acids, makes very desirable a method for their qualitative separation and, if possible, for their quantitative determination. The difference in their solubilities in various solvents or of the solubilities of the salts used in their preparation (1) is not very marked and causes lo...
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ژورنال
عنوان ژورنال: Acta Chemica Scandinavica
سال: 1969
ISSN: 0904-213X
DOI: 10.3891/acta.chem.scand.23-3023